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Allisure® AC-23- Powder stability testing
 
Dr Ronald R Culter testing Allisure® AC-23 stability in the laboratory

"We have developed a new method for testing the stability of standardised allicin extracts based on microbiological activity"This measurement is a genuine reflection of the ability of the test substance to kill pathogens and this methodology can also clearly demonstrate activity in the human body."

Previously established methods like HPLC are outdated and difficult to interpret as each testing laboratory uses different methodology and different standards and is only able to measure allicin release in a test sample. This bears no relation whatsoever to what actually happens in the human body. Any production system that relies on the activity of an acid sensitieve enzyme is

 
 
likely to fail significantly when actually given to human subjects. Our method is a direct measure of microbiological activity and each test sample of Allicin liquid, powder or cream is assayed  against a control strain of MRSA (methicillin resistant Staphylococcus aureus). We use approved methods to ascertain the "zone of inhibition" (British Society of Antimicrobial Chemotherapy) for each batch of allicin liquid,powder or cream. Zone size is directly related to allicin concentration and provided the ring of confiednce measures over 12mm then the test extract is deemed capable of destroying MRSA. This methodology will gain full validation when it is published in full in the medical literature later in 2004.
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In the meantime we have also used both HPLC and Mass Spectrometry,firstly to confirm the presence of allicin in the samples we have tested and then also to determine the relative concentration available over a period of 24 months having kept the samples under refrigeration (not frozen) for this period.
Hence the available concentration of allicin from each batch of Allisure powder does not vary significantly over a 2 year period. Recently data has been expanded to 3 years and there doesnot appear to be any significant degradation in activity since a clear zone of inhibition is still found.
Thus we are confident that the current legal requirement of a 2 year shelf life is fair for this type of garlic extract.
"We would not expect any other commercially available garlic extract to work as well as Allisure R. Simply because they either do not contain any allicin or they rely on your body making it, which often doesn't happen or sometimes produces a very low yield of allicin. Hence,no other product has any blinded published clinical data on anti-microbial activity".
 
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  Dr Ronald R Culter
PhD,MSc(Lond),MSc(Cranfield),BSc, MIBiol,FIBMS,DMS
Dept of Biosciences, University of East London
 


MATERIAL SAFETY DATA SHEET


“Allisure® AC-23” GARLIC POWDER

1] Product Name:
“Allisure® AC-23 ” Garlic Powder.
Contact

Allicin International Ltd Half House, Military Road
Rye , East Sussex , TN31 7NY, UK
Telephone ++44 (0) 1797 227959 
Fax ++44 (0) 1797 223404.
Email nopex@themutual.net

2] Product Identification   “Allisure® AC-23 ” GARLIC POWDER
Chemical name:   thio-2-propene-1-sulfinic- acid S-allyl ester
Tariff Number:   3004.50.40
CAS Number:   539-86-6
Formula:   C6H10OS2
3] Hazard Identification   Non hazardous
4] First Aid Procedures    
Inhalation:  

Remove to fresh air and seek medical attention if necessary

Skin Contact:  

Wash with soap and water. Remove contaminated clothing. If irritation persists or tissue damage is evident, seek medical advice.

Eye Contact:  

Immediately flush eyes with plenty of water or saline solution for at least 10 minutes. Seek medical advice

Ingestion:  

Rinse mouth with water. Give water to drink. Obtain medical advice. Do not induce vomiting.

5] Physical and Chemical Properties    
Appearance:   Powder
Colour:   White to off white
Density   ~ 0.6 to 0.7 gm/cc
pH:   ~ 4.00 to 6.00
Moisture Content   ~ 2% to 4%

CERTIFICATE OF ANALYSIS

Product: Allisure® AC-23 Powder
Date: 16 June 2006


Test Results

 
Batch Number  

L6160

Allicin   ~500ppm
Moisture content   2.7 g/100g
Average density (tapped)   0.682 g/cm  3
PH  

5.49 pH units

Yeast  

<10 cfu/g

Moulds  

<10 cfu/g

Enterobacteriaceae  

<10 cfu/g

E. Coli  

Absent in 0.1 g

Salmonella  

Absent in 1g

Listeria  

Absent in 1g

Staph Aureus  

<10 cfu/g

Total Viable Count   <10 cfu/g

Heavy Metal Analysis

   
Cadmium   <0.02 mg/kg dry wt
Lead   0.26 mg/kg dry wt
Arsenic   0.06 mg/kg dry wt
Mercury   <0.01 mg/kg dry wt
Date of manufacture   June 2006
     

N J Bennett 
Director
Allicin International Ltd

   

SPECIFICATION for Allisure® AC-23 ALLICIN POWDER

DATE

June 2006
PRODUCT NAME :   Allisure® AC-23 ALLICIN POWDER
PARAMETER   SPECIFICATION
Description:  

White to off white fine powder 
Odour: characteristic
Non GM Maltodextrin: carrier
Allicin: active agent

Heavy Metals:

 

 

Cadmium  

Less than 0.05 mg/kg dry wt

Lead  

Less than 0.50 mg/kg dry wt

Arsenic  

Less than 0.07 mg/kg dry wt

Mercury  

Less than 0.05 mg/kg dry wt

Microbiology:

 

 

Listeria  

Absent in 1 g

Salmonella  

Absent in 1 g

E.coli

  Less than 0.1 g
Staph aureus   Less than 20 cfu/g
Enterobacteriaceae  

Less than 10 cfu/g

TVC   Less than 10  4 cfu/g
Yeasts  

Less than 500 cfu/g

Moulds  

Less than 500 cfu/g

Pesticides   European Pharmacopeia
Other characteristics:    
Moisture (loss on drying)  

Typically 2 - 4 g/100g

Bulk Density   Typically 0.70 g/cm  3
pH   Typically 4 – 6
Allicin   max 300ppm

NON-GMO & NON IRRADIATION STATEMENT 

We include the instruction on all purchase orders that products should be NON-GMO and NON- IRRADIATED. We hold on file supplier statements on the GM status of their products. Every care is taken to supply the correct product, however this statement should not relieve the buyer of their own responsibility under due diligence to ensure the goods are suitable for the purpose for which they are intended.

   
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